As one important component of quality system in food and beverage industry, HACCP plays a very effective role in assuring food safety with the precondition that people can use it properly in which practice experience and structured food safety knowledge are the cores to arm people of production unit besides the principle. Policy, guideline and supervison from the government are the other side to assure the food safety in which food safety knowledge, supervising system, and effective legal system are the huge challenges.
Bacillus sporothermodurans cannot be killed by UHT which contributes the whole batch products' spoilages or unsterilities. Bacillus sporothermodurans producing highly heat-resistant spores (HRS) which may survive ultra-high temperature (UHT) treatment or industrial sterilization. Molecular typing showed a heterogeneous group of farm isolates (non-HRS strains), but a clonal group of UHT isolates from diverse European countries and other continents (HRS-clone) suggesting a common source. During a survey of Belgian dairy farms for the presence of potentially highly heat-resistant spore formers, high numbers of these spores were detected in filter cloth, green crop and fodder samples. The strain collection showed a high taxonomic diversity with 18 potentially new species and with Bacillus licheniformis and Geobacillus pallidus as predominating species overall. Seventeen B. sporothermodurans isolates were identified, mainly originating from feed concentrate. Heat resistance studies showed the UHT resistance of B. sporothermodurans spores present in industrially contaminated UHT milk, but a lower heat resistance of laboratory-grown strains (HRS and non-HRS). Hydrogen peroxide, used as sanitizer in the dairy industry, was found to induce higher heat resistance of laboratory-grown B. sporothermodurans strains to a certain level. This indicates that sublethal stress conditions may affect the heat resistance. By transmission electron microscopy, structural differences at the spore level were found between HRS and non-HRS strains. The data indicate that the attainment of extreme heat resistance is rather multifactorial.
How to clean round the bend removing completely any dairy residues in the milk or beverage transfer pipes which may be the growth bed or protection of microbes.
Where do the bacteria come? Using infection pattern, the question can be answered.
Pattern 1: logarithmic
Either the rate of infection at the source increases with time and/or substantial microbial growth takes place somewhere in the system.
One area that can be excluded/eliminated from such a scenario is faulty CIP or pre-sterilisation.
But leakage of paper tube, leakage of Valve 78, leakage of Aseptic tank, holding section of UHT and contaminated floater etc. have high probabilities.
Pattern 2: wash out
CIP/Sterilization failure linked bacteria residues often contribute such scenarios. Filling tube of Tetra Fino often give mixed cocci contamination if the tube is scratched by wrong cleaning brush, the seat of Valve A sometimes give endospores wash out if the manual clean is not carried out in a very long time. Because Escherichia can grow vigorously in organic residue, the poor CIP may result pure Escherichia flora.
Pattern 3:Complicate Wash out
Besides the cause found in wash out pattern, raw milk with poor quality such as more endospores, leakages in the product transfer pipeline, gasket leakages in the product pipeline, leaked aseptic chamber, upper cover of Tetra Fino, low consentration of H2O2, no tween80 added to H2O2, and leakages in cooling pipeling of aseptic air system in Tetra Fino may result such contamination pattern.
Pattern4/5: intermittent
•Probe leakage—seep into milk; •Operational fault—splicing PM; •Homoginizer fault resulted rough particles with spores; •Counter-Valve such as V78; •Gasket leakage or holding section leakages in UHT etc.
Pattern 6: consistency
Wrong operation such as selecting wrong sterilization program in UHT; Failure of equipment such as poor sealing, equipment components or equipment settings out of specification such as low sealing pressure, and poor quality milk with thermo resitant spores etc.
Pattern 7: Wave like
It is the combinition of intermittent pattern and consistent pattern.
Pattern 8: Random
Often it is caused by operational fault.
Pattern 9: Gradual increase
No bacteria multiplication, but reinfection increases. For example, the jaw system loses its pressure grdually. The white roller loses its pressure gradually.
How ro remove microbial contamination in aseptic production is always a challenge. If the contamination is very little, the survivors will be less when processing. Once contaminated by microbes in the milk, the speed of microbes mulplification will be higher than the light. The visual coagulation or spoilage usually resulted by more than 100 million bacteria. Keeping a good habit in production is always a must such as clean hands routinely, clean floor routinely etc.